This data set was collected from a ocean acidification minicosm experiment performed at Davis Station, Antarctica during the 2014/15 summer season. It includes: - description of methods for all data collection and analyses. - flow cytometry counts; autotrophic cells, heterotrophic nanoflagellates, and prokaryotes

Sampling Samples were collected on board the RSV Aurora Australis between 22 January and 17 February 2016. The cruise surveyed the region south of the Kerguelen Plateau including the Princess Elizabeth Trough and BANZARE Bank in a series of eight transects covering 8165 km. Plankton communities were collected at 45 conductivity temperature depth (CTD) stations and seven additional underway stations, with biological replicates collected at two stations (52 independent sites). Surface water was sampled from 4 plus or minus 2 m depth using the uncontaminated seawater line. Deep Chlorophyll Maximum (DCM, 10-74 m) water samples were obtained using 10 L Niskin bottles mounted on a Seabird 911+ CTD. Plankton communities were size-fractionated by sequentially filtering 10 L seawater through 25 mm 20 micron (nylon) and 5 micron filters (PVDF), and 0.45 micron Sterivex filters (PVDF). Filters were stored frozen at -80 °C. DNA extraction and high-throughput sequencing DNA was extracted from half of each filter using the MoBio PowerSoil DNA Isolation kit at the Australian Genome Research Facility (AGRF, Adelaide, Australia; http://www.agrf.org.au). The V4 region of the 18S rDNA (approximately 380 bp excluding primers) was PCR-amplified using universal eukaryotic primers from all extracts and sequenced on an Illumina MiSeq v2 (2 x 250 bp paired-end) following the Ocean Sampling Day protocol (Piredda et al. 2017). Amplicon library preparation and high-throughput sequencing were carried out at the Ramaciotti Centre for Genomics (Sydney, Australia). Sequence analysis, OTU picking and assignment followed the Biomes of Australian Soil Environments (BASE) workflow (Bissett et al. 2016). Taxonomy was assigned to OTUs based on the PR2 database using the ‘classify.seqs’ command in mothur version 1.31.2 with default settings and a bootstrap cut-off of 60%. OTUs representing any terrestrial contaminants (e.g. human) and samples with low sequencing coverage (less than 7000 reads) were removed from the dataset. The date of sea ice melt for each station was estimated from daily SSM/I-derived sea-ice spatial concentration from the National Snow and Ice Data Centre (NSIDC) at 25 x 25 km resolution. Days since melt was considered to be the number of days between the date on which sea ice concentration first fell below 15% and the date of sampling. Other environmental variables included are in situ chlorophyll a, as an indicator of biological production, and near-surface salinity (mean over the upper 10 m) as an indicator for recent sea ice melt. Both environmental measurements were taken from the associated CTD seawater samples. The surface chlorophyll a in seawater (1-2 L) collected in Niskin bottles was analysed by high performance liquid chromatography (HPLC, provided by Karen Westwood and Imojen Pearce, Australian Antarctic Division, doi:10.4225/15/5a94c701b98a8). Sampling times are given in UTC.

During the K-Axis marine voyage from mid Jan-late Feb 2016, a diverse range of sampling techniques were employed to collect specimens and data. Each sampling event was recorded by scientists and technical support staff in a logbook that was kept in the operations room on board the Aurora Australis. This is a PDF of the scanned original document, compiled on paper during the voyage. event_number: A unique event identifier in the log, in the order that the events were written down (usually but not always chronologically) event_type: The code defined and used by each research project to identify the types of equipment deployed or samples collected for an event. event_type_prefix: A non-mandatory prefix field used by some research projects to identify the type of an event event_type_number: A sequential number or alphanumeric-number combination defined and used by each research project to identify unique equipment deployment or sample collection events station_number: A universal (voyage-wide) station number used across all projects to identify a nominal lat/lon position defined during voyage planning leg: A nominally straight-line section of the voyage track defined during voyage planning. The voyage track was planned as a series of roughly N-S and E-W transects that intersected in some locations. Legs start at a station and continue through more stations to a vertex-station which is the start of the next leg. Legs are numbered consecutively. waypoint: A GPS waypoint used by Aurora Australis crew, AAD science technical support and researchers to identify target lat/lon positions in the voyage. Some waypoints correspond with station numbers. start_date_utc: The start date of the event in UTC start_time_utc: The start time of the event in UTC start_lat_deg: The latitude (whole degrees) of the vessel at the beginning of the event start_lat_min: The latitude (minutes) of the vessel at the beginning of the event start_lat_dec_deg: The latitude (decimal degrees) of the vessel at the beginning of the event start_lon_deg: The longitude (whole degrees) of the vessel at the beginning of the event start_lon_min: The longitude (minutes) of the vessel at the beginning of the event start_lon_dec_deg: The longitude (decimal degrees) of the vessel at the beginning of the event end_date_utc: The end date of the event in UTC end_time_utc: The end time of the event in UTC end_lat_deg: The latitude (whole degrees) of the vessel at the end of the event end_lat_min: The latitude (minutes) of the vessel at the end of the event end_lat_dec_deg: The latitude (decimal degrees) of the vessel at the end of the event end_lon_deg: The longitude (whole degrees) of the vessel at the end of the event end_lon_min: The longitude (minutes) of the vessel at the end of the event end_lon_dec_deg: The longitude (decimal degrees) of the vessel at the end of the event remarks: Comments/remarks written by researchers when completing the paper log

During the K-Axis marine voyage from mid Jan-late Feb 2016, a diverse range of sampling techniques were employed to collect specimens and data. Each sampling event was recorded by scientists and technical support staff in a logbook that was kept in the operations room on board the Aurora Australis. This is a direct digital copy/transcription of the paper logbook. event_number: A unique event identifier in the log, in the order that the events were written down (usually but not always chronologically) event_type: The code defined and used by each research project to identify the types of equipment deployed or samples collected for an event. event_type_prefix: A non-mandatory prefix field used by some research projects to identify the type of an event event_type_number: A sequential number or alphanumeric-number combination defined and used by each research project to identify unique equipment deployment or sample collection events station_number: A universal (voyage-wide) station number used across all projects to identify a nominal lat/lon position defined during voyage planning leg: A nominally straight-line section of the voyage track defined during voyage planning. The voyage track was planned as a series of roughly N-S and E-W transects that intersected in some locations. Legs start at a station and continue through more stations to a vertex-station which is the start of the next leg. Legs are numbered consecutively. waypoint: A GPS waypoint used by Aurora Australis crew, AAD science technical support and researchers to identify target lat/lon positions in the voyage. Some waypoints correspond with station numbers. start_date_utc: The start date of the event in UTC start_time_utc: The start time of the event in UTC start_lat_deg: The latitude (whole degrees) of the vessel at the beginning of the event start_lat_min: The latitude (minutes) of the vessel at the beginning of the event start_lat_dec_deg: The latitude (decimal degrees) of the vessel at the beginning of the event start_lon_deg: The longitude (whole degrees) of the vessel at the beginning of the event start_lon_min: The longitude (minutes) of the vessel at the beginning of the event start_lon_dec_deg: The longitude (decimal degrees) of the vessel at the beginning of the event end_date_utc: The end date of the event in UTC end_time_utc: The end time of the event in UTC end_lat_deg: The latitude (whole degrees) of the vessel at the end of the event end_lat_min: The latitude (minutes) of the vessel at the end of the event end_lat_dec_deg: The latitude (decimal degrees) of the vessel at the end of the event end_lon_deg: The longitude (whole degrees) of the vessel at the end of the event end_lon_min: The longitude (minutes) of the vessel at the end of the event end_lon_dec_deg: The longitude (decimal degrees) of the vessel at the end of the event remarks: Comments/remarks written by researchers when completing the paper log transcribe_comments: Comments/remarks made by the transcriber when the log was digitised

During the K-Axis marine voyage from mid Jan-late Feb 2016, a diverse range of sampling techniques were employed to collect specimens and data. Each sampling event was recorded by scientists and technical support staff in a logbook that was kept in the operations room on board the Aurora Australis. This is a direct digital transcription of the paper logbook with interpolated lat/lon from underway data to supplement start times as recorded in the log. The method used to obtain the supplementary position is described in the associated eventlog_matchup.html file. event_number: A unique event identifier in the log, in the order that the events were written down (usually but not always chronologically) event_type: The code defined and used by each research project to identify the types of equipment deployed or samples collected for an event. event_type_prefix: A non-mandatory prefix field used by some research projects to identify the type of an event event_type_number: A sequential number or alphanumeric-number combination defined and used by each research project to identify unique equipment deployment or sample collection events station_number: A universal (voyage-wide) station number used across all projects to identify a nominal lat/lon position defined during voyage planning leg: A nominally straight-line section of the voyage track defined during voyage planning. The voyage track was planned as a series of roughly N-S and E-W transects that intersected in some locations. Legs start at a station and continue through more stations to a vertex-station which is the start of the next leg. Legs are numbered consecutively. waypoint: A GPS waypoint used by Aurora Australis crew, AAD science technical support and researchers to identify target lat/lon positions in the voyage. Some waypoints correspond with station numbers. start_date_utc: The start date of the event in UTC start_time_utc: The start time of the event in UTC start_lat_deg: The latitude (whole degrees) of the vessel at the beginning of the event start_lat_min: The latitude (minutes) of the vessel at the beginning of the event start_lat_dec_deg: The latitude (decimal degrees) of the vessel at the beginning of the event start_lon_deg: The longitude (whole degrees) of the vessel at the beginning of the event start_lon_min: The longitude (minutes) of the vessel at the beginning of the event start_lon_dec_deg: The longitude (decimal degrees) of the vessel at the beginning of the event end_date_utc: The end date of the event in UTC end_time_utc: The end time of the event in UTC end_lat_deg: The latitude (whole degrees) of the vessel at the end of the event end_lat_min: The latitude (minutes) of the vessel at the end of the event end_lat_dec_deg: The latitude (decimal degrees) of the vessel at the end of the event end_lon_deg: The longitude (whole degrees) of the vessel at the end of the event end_lon_min: The longitude (minutes) of the vessel at the end of the event end_lon_dec_deg: The longitude (decimal degrees) of the vessel at the end of the event remarks: Comments/remarks written by researchers when completing the paper log transcribe_comments: Comments/remarks made by the transcriber when the log was digitised utc: The start date and time of the event in UTC start_lon_dec_deg_interp: The latitude (decimal degrees) of the vessel at the beginning of the event interpolated from the vessel underway data start_lat_dec_deg_interp: The longitude (decimal degrees) of the vessel at the beginning of the event interpolated from the vessel underway data